A multiple variable-angle light-scattering detector for gel permeation chromatography
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چکیده
منابع مشابه
A Multiple Variable-Angle Light Scattering Detector for Gel Permeation Chromatography
A light scattering detector has been designed and constructed for use with gel permeation chromatographs. The detector measures light scattered from the eluting sample simultaneously at nine scattering angles, and is connected to a computer-controlled display which exhibits the angular dependence of the scattering in real time while the sample is eluting. Use of the light-scattering detector in...
متن کاملUse of low-angle laser light scattering with gel permeation chromatography for the molecular mass determination of biomolecules.
486 Use of low-angle laser light scattering with gel permeation chromatography for the molecular mass determination of biomolecules K. Jumel,* P. Br0wne.t and J. F. Kennedy# *Chembiotech Ltd, Institute of Research and Development, Vincent Drive, Edgbaston, Birmingham B I5 2sQ, U.K.; tLDC Analytical, Diamond Way, Stone Business Park, Stone, Staffs STI 5 OHH, U.K. and $Research Laboratory for the...
متن کاملGel permeation chromatography--multi-angle laser light scattering characterization of the molecular mass distribution of 'Pronova' sodium alginate.
510 Conclusions We have shown evidence for the bridging flocculation of PSL by p-LG. The change in surface charge of the PSL spheres resulting from adsorption of p-LG molecules correlates with the formation of aggregates bridged by protein molecules. To develop models of the kinetics of bridging flocculation, some measurements of aggregation as a function of time and temperature have been carri...
متن کاملDetection of serum proteins by high-pressure gel-permeation chromatography with low-angle laser light scattering, compared with analytical ultracentrifugation.
Human sera were subjected to analytical ultracentrifugation and "high-pressure" gel-permeation chromatography on a system of combined TSK Gel G5000 PW and G3000 SW columns. The chromatographic method produced remarkably superior resolution of the proteins, especially those exceeding 100 000 Da. We calculated the molecular masses of eluted fractions on the basis of their detection by low-angle l...
متن کاملHeat-induced aggregation of recombinant erythropoietin in the intact and deglycosylated states as monitored by gel permeation chromatography combined with a low-angle laser light scattering technique.
Aggregate formation of recombinant human erythropoietin (r-EPO) on heat-treatment was followed by gel permeation chromatography combined with a low-angle laser light scattering technique under various conditions with respect to pH and salt concentration in order to provide basic knowledge about the change strictly required to be monitored for medicinal proteins. When heated at 60 degrees C at n...
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ژورنال
عنوان ژورنال: Journal of Research of the National Institute of Standards and Technology
سال: 1991
ISSN: 1044-677X
DOI: 10.6028/jres.096.007